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The human beta-interferon gene enhancer is under negative control.

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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei. Structure of a chromosomal gene for human interferon beta.

Support Center Support Center. Establishment of a permanent cell line HEL-R66 from human embryonic lung cells with high susceptibility to viruses. The 5′-flanking sequence of human interferon-beta 1 gene is responsible for viral induction of transcription.

Changes of chromatin conformation around mouse interferon-beta gene associated with induction of interferon synthesis. Author information Copyright and License information Disclaimer. Reversible silencing of enhancers by sequences derived from the human IFN-alpha promoter.

BK viral enhancer element and a human cellular homolog. IRF-1 may also be involved in silencing the function of the SV40 enhancer juxtaposed to the regulatory elements in uninduced cells. Intracytoplasmic phosphorylation sites of Tac antigen p55 are not essential for the conformation, function, and regulation of the human interleukin 2 receptor.


A rapid and sensitive assay system for bacterial gpt activity in transfected mammalian cells.

A nucleotide promoter segment from an IFN-alpha gene renders an unrelated promoter inducible by virus. High efficiency DNA-mediated transformation of primate cells. Open in a separate window. Selection for animal cells that express the Escherichia coli gene coding for xanthine-guanine phosphoribosyltransferase. Delimitation and properties of DNA sequences required for the regulated expression of human interferon-beta gene.

Tissue-specific expression of the human growth hormone gene is conferred in part by the binding of a specific trans-acting factor. Human beta-interferon gene expression is regulated by an inducible enhancer element. Controlled transcription of a human alpha-interferon gene introduced into mouse L cells. National Center for Biotechnology InformationU. Associated Data Supplementary Materials.

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Regulation of gene expression by interferons: A nuclear factor that binds to a conserved sequence motif in transcriptional control elements of immunoglobulin genes. Short and long range activation by the 46100 enhancer. Interaction of positive and negative regulatory proteins in the galactose regulon of yeast.

In this study, we describe a nuclear factor, termed interferon regulatory factor-1 IRF-1that is involved in the transcription of IFN-beta and possibly other genes. Please review our privacy policy. Biochemistry of interferons and their actions. Human embryonic lung cells HEL-R66 are of monkey origin. Affinity purification of sequence-specific DNA binding proteins. Our data suggest that IRF-1 participates in the transient formation of an induction-specific complex es with the regulatory elements.


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Sequencing end-labeled DNA with base-specific chemical cleavages. Abstract Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines.

Transcription of the human interferon-beta IFN-beta gene is induced by a variety of agents such as viruses, dsRNA and some cytokines. Identification of two distinct regulatory regions adjacent to the human beta-interferon gene. Regulation of cytokine gene expression. Images in this article Irg on p. Heat shock factor is regulated differently in yeast and HeLa cells.